Rival Haiti relief workers Sean Penn and Wyclef Jean were involved in a tense confrontation at a top New York music venue after trading a series of bitter public insults.

Penn and Wyclef have been at each other’s throats after Penn criticized Wyclef’s ill-fated bid to become president of Haiti, blasted him as a “personality that threatens to create a new swell of social unrest in a plagued country” and accused him of deserting the country when it most needed help.

The former Fugee then lashed back by saying: “I got a message for Sean Penn: Maybe he ain’t see me in Haiti because he was too busy sniffing cocaine.”

Penn’s rep shot back at Jean’s comments as “highly irresponsible and false.”

But Penn and Wyclef bumped into each other at B.B. King Blues Club & Grill in Times Square early Saturday morning after they arrived separately to see Haitian band T-Vice, and locked themselves in a backstage dressing room for a 30-minute argument before emerging, having made peace.

WATCH SEAN PENN AND WYCLEF ADDRESS THE CROWD

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A source told us: “They were both in the venue, and when they saw each other at around 1 a.m., they started a confrontation. Things seemed pretty tense, and everyone around them was nervous. They then asked staff if they could go somewhere private, so they were led into a dressing room backstage where they could be alone and shut the door. They weren’t yelling and screaming, but they had it out and aired their differences for 30 minutes. When they came out they hugged each other, they went onstage and stood side-by-side.”

Drink in hand, Penn then took the mike and gestured at Wyclef and Michel Martelly, who is running for president of Haiti and urged the crowd to support them. He said: “In Haiti, we have got an opportunity as Americans whether you are from Haitian diaspora or pr – – ks like me . . . These two men beside me will lead the way if you support them . . . let’s move on for some fun.”

Also watching was fellow Fugee Pras, actress Paz de la Huerta, actor Jeffrey Wright and nightlife queen Amy Sacco.

Wyclef’s reps didn’t get back to us. Penn’s rep declined to comment about Wyclef.