Yet another showdown took place at the Meatpacking District’s Double Seven on Monday, this time between club doyenne Amy Sacco and feminist punk pioneer JD Samson.

Sources told us Samson, a member of the electro band Le Tigre and a DJ, was offended when she thought Bungalow 8 owner Sacco made a “condescending” comment about her.

Sources said Sacco made an innocent comment about Samson — the moustache-sporting, lesbian co-founder of performance art group “Dykes Can Dance” — near the DJ booth. Samson took offense, and retaliated by calling Sacco a “grandma.”

“Amy said something that was taken the wrong way, and the DJ reacted strongly and started making remarks on Twitter,” a source told us, adding that Samson also had words with a different guest about her choice of music and her sexuality.

While witnesses insisted Sacco did not make any anti-gay comments, Samson fired off emotional posts on her feed yesterday: “Gay bashed at my own dj night.” She later tweeted, “I had a hard night. the reality of my body in this society. and how i fit in. today i take to the streets to demand equality and change.”

Samson’s management also fired off an email of complaint to bosses of Double Seven — the scene of the infamous February “battle royale” involving Monaco’s Pierre Casiraghi.

Sacco says she merely spoke with Samson in the DJ booth, discussing the disco music Samson wanted to play. She said, “I told her to pump it up, and it would be fine. The only other thing she said to me was that she was an international DJ, before she said, ‘Do you know I am a girl?’ That was the extent of it.”

“This is clearly an attempt to get press on her behalf at my expense,” added Sacco, who plans to reopen Bungalow as a gastropub on West 16th Street in June. “I am extremely disappointed and saddened it has come to this level.” Despite her complaints on Twitter, Samson said, “This story is completely false.”